![primordia resolution primordia resolution](https://www.imlgames.com/wp-content/uploads/photo-gallery/thumb/primordia-03.jpg)
Regulatory genes expressed in the abaxial domain suppress those expressed in the adaxial domain and vice versa. These genes encode transcription factors and small RNAs, and their domain-specific expression patterns are required for adaxial-abaxial asymmetric cell differentiation and lamina expansion.
![primordia resolution primordia resolution](https://www.metal-archives.com/images/4/6/0/2/460200.jpg)
Opposite to the original proposal, instead of a signal derived from the meristem, we show that a signaling molecule is departing from the primordium to the meristem to promote robustness in leaf patterning.Įxtensive molecular genetic studies of more than a decade have identified a transcriptional regulatory network containing several adaxially or abaxially expressed leaf abaxial- and adaxial-promoting genes ( 1– 6). Our results provide an explanation for the hypothetical meristem-derived leaf polarity signal. Furthermore, we show that auxin flow from emerging leaf primordia to the shoot apical meristem establishes the low auxin zone, and that this auxin flow contributes to leaf polarity. The auxin signal is mediated by the auxin-responsive transcription factor MONOPTEROS (MP), whose constitutive activation in the adaxial domain promotes abaxial cell fate. We also demonstrate that this adaxial low auxin domain contributes to leaf adaxial-abaxial patterning. Here we show the existence of a transient low auxin zone in the adaxial domain of early leaf primordia. It has long been proposed that the stem cells at the plant shoot apex produce a signal, which promotes leaf adaxial-abaxial (dorsoventral) patterning. Stem cells are responsible for organogenesis, but it is largely unknown whether and how information from stem cells acts to direct organ patterning after organ primordia are formed.